Posts not found

PHOSPHOLIPASE A2

PHOSPHOLIPASE A2 (PA2) is an enzyme derived from porcine pancreas. It serves as a catalyst in the hydrolysis of the fatty acid in the second position of phospholipids/lecithin.
This process results in the conversion of approximately 70% of the lecithin found in egg yolk to lysolecithin, which has much better emulsifying properties than lecithin.

Neova Technologies PLA2-formula

ADVANTAGES & BENEFITS

A key benefit associated with using Neova Technologies Inc. pancreatic enzymes lies in the full range of services, including both technical and applications support that Neova provides. Neova works with its customers to meet their unique product specifications.

Raw materials are sourced from government-certified facilities. All extraction and refining is done under cGMP guidelines for active pharmaceutical ingredients (API).

Neova’s PHOSPHOLIPASE A2 (PA2)  complies with the recommended purity requirements for food grade enzymes provided by the joint FAO/WHO Expert Committee on Food Additives (JECFA), and the Food Chemical Codex (FCC).

APPLICATIONS

PHOSPHOLIPASE A2 (PA2) can be used in the production of heat-stable egg yolk. Enzyme modified egg yolk offers a number of benefits in various food systems, including mayonnaise, sauces, dressings, baking and ice cream.

One benefit provided by enzyme-modified egg yolk is better emulsifying properties than untreated egg yolk; this functionality serves to stabilize otherwise incompatible ingredients.

Modified egg yolk also demonstrates a high degree of stability at elevated temperatures (i.e. 70˚- 80˚C) compared to untreated yolk that has little tolerance for temperatures above 60˚C. Modified egg yolk treated with Phospholipase A2 PA2 has demonstrated superior stability at retort temperatures above 150˚C when used in certain food systems (i.e., mayonnaise). This stability allows for the incorporation of pasteurization into processing, which is critical for microbial quality and which also contributes to increased shelf life.

Increased viscosity is another benefit offered by modified egg yolk, which is particularly important in the production of mayonnaise.

FOOD APPLICATIONS

Phospholipase A2 is use extensively as a processing aid in many food applications, including:

Enzymatic degumming of vegetable oil;
Low fat cheese and dairy production;
Improving the heat stability and emulsifying properties of egg yolk and soy products;
Promoting antibacterial activity against both GRAM-positive and GRAM-negative bacteria;
Preventing oiling out during cheese production, thereby increasing yield;

PHARMACEUTICALS

Phospholipase A2 has a variety of applications in drug delivery systems.

PURITY & ACTIVITY
OPTIMAL RANGES

Figure 1:

Residual Activity vs pH at 50°C

[weblator_chart id=”1″]

Figure 2:

Activity vs Temperature at pH8.5

[weblator_chart id=”2″]

Figure 3:

PA2 Stability at different pH values

[weblator_chart id=”3″]

Figures 1 and 2 demonstrate the effect of pH and temperature on the activity of PA2 .

Figure 3 demonstrates the effect of different pH values at different temperatures on the enzymatic activity of PHOSPHOLIPASE A2 .

REGULATORY STATUS

PHOSPHOLIPASE A2 (PA2) preparations meet both the current Food Chemical Codex (FCC) specifications and the Joint WHO/FAO Expert Consultation on Food Additives (JECFA) Compendium of Specifications for Food Grade Enzyme Preparations.

  • E.C. (Enzyme Classification) Numbers: Phospholipase A (porcine pancreatic) – C 3.1.1.4
  • CAS (Chemical Abstracts Registry) Number: Phospholipase – 9001-84-7

Non Warranty: The information contained herein is provided in good faith and, to the best of our knowledge, is true and correct. However, no warranty or guarantee is implied or inferred and the information may be subject to change without further notice. Neova Technologies Inc.’s products are sold with the understanding that the purchaser will conduct tests to determine the suitability of these products for their particular use.