OVALBUMIN The standard in proteomic investigation of composition, physical properties, and structure of proteins – in high purity crystalline form.

Neova -Ovalbumin-label

OVALBUMIN the effective carrier and stabilizer protein for immunological and biochemical studies

Neova -Ovalbumin-label

OVALBUMIN for the detection of anti-hemoglobin monoclonal antibodies in enzyme-linked immunosorbent assays (ELISA)

Neova -Ovalbumin-label

OVALBUMIN the standard enzyme in SDS-PAGE molecular weight determination and size exclusion chromatography

Neova -Ovalbumin-label

OVALBUMIN

OVALBUMIN is the predominant protein in egg white, comprising approximately 54% of the total protein. It is one of only two pure proteins that can adequately meet nutritional requirements for amino acids. OVALBUMIN was first isolated through successive salt precipitations in 1889, and the procedure was later improved in the first part of the 20th century.

The standard purification procedures are labour intensive and difficult to mechanize, which meant that large-scale production of pure OVALBUMIN was not feasible. Neova Technologies has developed a purification procedure using chromatography techniques. As a result it is able to produce extremely pure OVALBUMIN in commercial volumes. OVALBUMIN is very similar in amino acid content to bovine serum albumin (BSA) (see Table below) and can be an excellent substitute for BSA.

 Table: Comparison of Amino Acids Between Albumins*
Amino Acids Ovalbumin Lactalbumin BSA
Lysine 6.6 11.5 12.8
Histidine 12.3 2.9 4.0
Arginine 5.9 1.2 5.9
Aspartic Acid 9.4 18.7 10.9
Threonine 4.5 5.5 5.8
Serine 8.1 4.8 4.2
Glutamic Acid 16.1 12.9 16.5
Proline 3.6 1.5 4.8
Glycine 3.2 3.2 1.8
Alanine 5.8 2.1 6.3
Half Cystine 2.4 6.4 6.5
Valine 7.1 4.7 5.9
Methionine 4.9 1.4 0.8
Isoleucine 7.0 6.8 2.6
Leucine 10.1 11.5 12.3
Tyrosine 3.9 5.4 5.1
Phenylalanine 5.4 4.5 6.6
Tryptophan 1.2 5.3 0.6

 

* Grams per 100 grams of protein.

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Advantages and benefits

As one of the largest manufacturers and suppliers worldwide of egg white proteins, Neova Technologies advanced extraction and purification processes yield products that are consistently chosen over other competitive products. All extraction and refining is done under cGMP guidelines for active pharmaceutical ingredients (API).

A key benefit associated with using Neova Technologies OVALBUMIN lies in the full range of services, including both technical and applications support that Neova Technologies provides. Neova Technologies works with its customers to meet their unique product specifications.

An important advantage to using Neova Technologies OVALBUMIN is the elimination of the risk of disease associated with bovine and human derived albumins. This is because OVALBUMIN originates from a safer starting material than the other albumins and is processed to a pure form under more sanitary conditions.

Two grades of OVALBUMIN are available in a crystalline, lyophilized (freeze-dried), powder:

  • OVALBUMIN 90: minimum 90% protein
  • OVALBUMIN 98: minimum 98% protein (superior to 5X crystalline)

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Applications

OVALBUMIN is a key reference protein for immunization and biochemical studies. Some of its well-established uses include:

  • Investigation of composition, physical properties & structure of proteins
  • Blocking agent in immunohistochemistry and in Western blots of proteins in solution;
  • Detection of anti-hemoglobin monoclonal antibodies in immunosorbent assays (ELISA);
  • Standard Protein in molecular weight determination by SDS-PAGE & size exclusion chromatography. antibodies;

In addition, OVALBUMIN may be used in cell culture systems and in the diagnostic industry to stabilize enzymes and hormones that would otherwise lose their functional integrity. It is also ideal as a reference protein for both immunological and biochemical studies, where it serves as an effective carrier and as a stabilizer protein.

Non Warranty: The information contained herein is provided in good faith and, to the best of our knowledge, is true and correct. However, no warranty or guarantee is implied or inferred and the information may be subject to change without further notice. Neova Technologies Inc.’s products are sold with the understanding that the purchaser will conduct tests to determine the suitability of these products for their particular use.